In addition to hyperproliferation of keratinocytes, psoriasis is characterized by pronounced
leukocytic infiltration. In contrast to the epidermal localization of neutrophils and T
lymphocytes, macrophages are almost exclusively restricted to the dermal compartment. By
immunohistologic analysis, these dermal macrophages were mainly encountered in the
papillary dermis and arranged along the rete ridges in close proximity to proliferating
keratinocytes. Monocyte chemoattractant protein (MCP-1) anti-sense RNA probes yielded …

MATERIALS AND METHODS Selection and Preparation of Skin Sections Fresh incisional
biopsy specimens of lesional and non-lesional skin of patients with untreated, longstanding
psoriasis were taken under local anesthesia (n= 9). For control purposes, biopsies were
taken from five healthy volunteers. The material was snap-frozen and 4-um cryostat sections
were used for immunohistology and in situ hybridization. Immunohistology For
immunohistology the following monoclonal antibodies (MoAbs) were employed: MoAb anti …