The main focus of this study is to check if there is any significant difference among Iranian advanced males and females EFL learners’ reading fluency. To fulfil this objective, 20 males and 20 females were selected based on an Oxford Quick Placement Test. Each participant read two reading passages and their voices recorded for further analysis. Later, two raters analyzed the recorded voices based on accuracy measurement criteria. The researcher checked inter-rater reliability for reading fluency and it was 0.986. After analyzing the data via independent samples t-test, the results revealed that female participants performed better than the males in terms of reading fluency. Conclusions are made concerning the applicability of these results to the Iranian EFL contexts.