Fire might occur under natural conditions in the Pantanal of Brazil; however, with climate change, severe periods of drought potentiated the devasting fires in 2020, resulting in substantial wildlife loss. Considering that mammal communities are strongly affected by fire and habitat alterations, the aim of this study was to evaluate possible differences in mammal diversity and the number of records before and one year after the fire in one region of the Pantanal of Mato Grosso, Brazil (Parque SESC Baía das Pedras - PSBP). The data collection was performed using camera trapping between 2015 and 2017 and 2021, together with visual field observations in PSBP. We observed that the mammal assemblage composition was similar before and one year after the fire. Four species were more or less frequent in burned areas than in unburned ones. Since the fire was controlled in this area, avoiding its total destruction, and the species that fled from the surrounding areas, which were completely burnt, might be using PSBP as a refuge while the vegetation recovers elsewhere. Therefore, the PSBP might have contributed to protecting mammal species after the fire and maintaining and conserving biodiversity on a regional scale in the Pantanal of Mato Grosso, Brazil.