Groundnut is an important oilseed crop of India, grown extensively in various parts of the country. Stem rot caused by (Sclerotium rolfsii Sacc.) is the major disease, affecting the groundnut cultivation across the India and worldwide. The main aim of this study was to evaluate the antagonistic potentiality of T. harzianum (Th-BKN), T. viride (Tv-BKN), P. fluorescens (Pf-BKN) and B. subtilis (Bs-BKN) against stem rot of groundnut in field conditions. Among the treatment, the treatment module consisting with soil application with T. harzianum (Th-BKN)@ 10 kgha-1+ FYM@ 10 tonn. ha-1 gave highest root length, shoot length, fresh weight, dry weight, disease control, pod yield in kgha-1 and lowest disease incidence (9.90 cm, 21.05 cm, 222.60 gm, 65.20 gm, 86.30%, 2173 kgha-1 and 7.51%) followed by in module consisting with treatment of soil application with T. viride (Tv-BKN)@ 10 kgha-1+ FYM@ 10 tonn. ha-1 (9.10 cm, 20.03 cm, 202 gm, 55.03 gm, 79.78%, 1933 kgha-1 and 11.08%,) respectively.