This paper has a twofold objective: mapping land cover classes from Landsat-8 (OLI) focusing mainly on date palm plantations in Abu Dhabi Emirate (UAE). Second, assessing the above ground biomass (AGB) and carbon sequestered (CS) of those plantations. Data from Landsat-8 OLI were used to classify and map date palm plantations (DP) in the study area. Hybrid classification approach was adopted. Results were validated using existing ancillary data and field checks. Mapping accuracy was acceptable at this scale. DP were mapped with an overall accuracy of 81.71% which was considered high in similar conditions of drylands, while the overall kappa statistics was estimated at 80. 94%. DP map was used as an input to the developed remotes sensing-based biomass model assessment to estimate AGB and CS in the Emirate of Abu Dhabi. The total AGB and CS for mature date palm plantations in Abu Dhabi were estimated respectively at: 613,224.23 and 330,343.89 tons.