… binary protein-protein interaction (PPI) mapping based on optimally combining assays and/or
assay versions to maximize … recovers as many PPIs as 10 distinct assays combined. Thus, …

… To maximize sensitivity we used three Y2H assay versions (Fig. 1b, Supplementary Tables
3, 4) that showed good sensitivity and low false-positive rates when benchmarked against …

… binary protein-protein interaction (PPI) mapping based on optimally combining assays and/or
assay versions to maximize … recovers as many PPIs as 10 distinct assays combined. Thus, …

… By performing ten independent screens testing a total of 36 million binary interaction …
maximizing for the number of positively scoring known interactions and minimizing for the number …

… interaction pairs in assay version 1, screen 1; 1029 in assay … approaches to binary interaction
mapping, together with … a next-generation reference binary interactome for Drosophila. The …

… The large number of assays that need to be performed to … , followed by maximizing the
number of interaction assays. … interactions to a more rigorous interaction testing. For the decision …

… To systematically map direct virus-host PPIs with binary interaction assays, two strategies
can be applied in … Maximizing binary interactome mapping with a minimal number of assays. …

… interactome. Typically, when performed under conditions that limit the detection of negative
control proteins, state-of-the-art binary PPI assays … threshold that maximizes the detection of …

… spoke-expanded binary relationships. Dots represent the date when the interaction was
curated. (B) Amount of cumulative experimental evidence associated with unique binary pairs, …

… This indicates that PCAs interrogate a different space of the interactome than biochemical
and Y2H assays and highlights the need of using complementary approaches for PPI mapping…