Characterizing immune repertoires by high throughput sequencing: strategies and applications

JJA Calis, BR Rosenberg - Trends in immunology, 2014 - cell.com
… On account of these similarities, hereafter we refer to BCRs and TCRs … immune memory.
As such, immunologists have developed a variety of techniques in attempts to measure diversity

High-throughput sequencing-based immune repertoire study during infectious disease

D Hou, C Chen, EJ Seely, S Chen, Y Song - Frontiers in immunology, 2016 - frontiersin.org
… and TT, have revealed dynamic changes in the size and diversity of antibody repertoires
before and after antigen stimulation (41, 90, 95, 96). Comparison of post-vaccination responses …

Advanced methodologies in high-throughput sequencing of immune repertoires

S Friedensohn, TA Khan, ST Reddy - Trends in biotechnology, 2017 - cell.com
… In the past, empirically measuring the enormous diversity of lymphocyte repertoires was
relegated to qualitative approaches, such as complementarity determining region 3 (CDR3) …

Qualifying high-throughput immune repertoire sequencing

N Niklas, J Pröll, J Weinberger, A Zopf, K Wiesinger… - Cellular …, 2014 - Elsevier
… considering VDJ combination and clone size. Spectra were … single sequence but the immune
repertoire and its diversity. … , AZ, KW performed high-throughput sequencing; KK performed …

T cell repertoire following kidney transplantation revealed by high-throughput sequencing

L Lai, L Wang, H Chen, J Zhang, Q Yan, M Ou… - Transplant …, 2016 - Elsevier
… limit the faithful measurement of TCR diversity after kidney … compared to the NC group (n
= 10) to dissect the recovery of the … the entire diversity of the immune repertoire at sequence-…

… heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using highthroughput sequencing

L Lai, X Zhou, H Chen, Y Luo… - Experimental and …, 2019 - spandidos-publications.com
… used to measure the diversity of B-cell populations (15). For … SE is a measure of diversity in
the immune repertoire, with a … limited sample size of the present study, most differences were …

High-throughput sequencing of the T-cell receptor repertoire: pitfalls and opportunities

JM Heather, M Ismail, T Oakes… - Briefings in …, 2018 - academic.oup.com
… High-level processing can measure the diversity of the … and comparing different repertories,
measuring diversity, annotating … of the total diversity or richness of the human immune system …

Computational estimation of clonal diversity in autoimmunity

ZK Tuong, R van der Merwe… - Immunology and Cell …, 2024 - Wiley Online Library
… in high-throughput sequencing, single-cell technologies and bioinformatics, the ability to
measure the diversity of the immune … By contrast, β diversity measures the difference in immune

The promise and challenge of high-throughput sequencing of the antibody repertoire

G Georgiou, GC Ippolito, J Beausang, CE Busse… - Nature …, 2014 - nature.com
… restricts the diversity of the library to very few antibody clones with … size of the antibody
repertoire, variable sampling depth, transcriptional differences among B-cell subsets, sequencing

A bioinformatic framework for immune repertoire diversity profiling enables detection of immunological status

V Greiff, P Bhat, SC Cook, U Menzel, W Kang… - Genome medicine, 2015 - Springer
… , through the advent of high-throughput sequencing (HTS), … exceeds the size of published
immune repertoire HTS … framework and high-throughput repertoire sequencing, we are …