This study was conducted in September 2014 to determine the effects of metal accumulation on the Odanata larvae which is a freshwater macroinvertebrate. Polluted area in the lower part of the mine founded on Umurbey Stream (Çanakkale, Turkey) and unpolluted area in the upper part of it are defined as the sampling stations. In this study, GSH (Glutathione), TBARS levels and Na+, K+-ATPase activity were measured after the determination of metal accumulation (Cd, Cu, Fe, Pb, Zn) in the water and in the Odanata larvae, Ischnura elegans (Vander Linden, 1820). There was a decrease in Na+, K+-ATPase activity; although the increase in GSH and TBARS levels in organisms sampled from polluted area. These results indicate that; metal accumulation caused to oxidative stress in Odanata larvae I. elegans and this organism reacted by running the compensate mechanisms for it.