operated for 382days to evaluate the process performance and methanogenic population
dynamics. A real-time quantitative PCR (QPCR) was used to detect and quantify the 16S
rRNA gene concentrations of the domain Archaea, the four methanogenic orders, and the
two aceticlastic families. Extended intervals of consistently stable and efficient wastewater
treatment with a final hydraulic retention time (HRT) of 3.5 days were sustained. A high …