Methods for catalyzing CH insertion reactions using heme enzymes are described. The
present disclosure provides a method for producing a CH insertion product comprising
providing an substrate having an spä-hybridized C–H bond, a carbene precursor such as a
diazo reagent, and a heme enzyme, and admixing the components in a reaction for a time
sufficient to produce the CH insertion product. Heme enzyme variants useful for carrying out
in vivo and in vitro C–H insertion reactions, as well as expression vectors and host cells …