Methods of culturing mesenchymal stromal cells
S VISWANATHAN, SJ BHATT, A Gomez-Aristizabal - 2018 - Google Patents
There is described herein a method for culturing mesenchymal stromal cells (MSCs)
comprising: a) providing a population comprising mesenchymal stromal cells; and b)
enhancing the population under conditions that allow the MSCs to loosely self-aggregate
and in the presence of cytokine to produce culture engineered MSCs (ce-MSCs). In an
embodiment, the cytokine is present at 0.1-15 ng/ml.
comprising: a) providing a population comprising mesenchymal stromal cells; and b)
enhancing the population under conditions that allow the MSCs to loosely self-aggregate
and in the presence of cytokine to produce culture engineered MSCs (ce-MSCs). In an
embodiment, the cytokine is present at 0.1-15 ng/ml.
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