… the present invention provides new and novel approaches for such controlled systems,
including a system for integrating vascular cells and organ cells to reproduce functional
units of tissues and organs in vitro. a microfluidic system for generating compartmentalized
microenvironments of tissues and organs in vitro and for independently perfusing the
compartments is herein disclosed. … a method for reproducing a functional unit of an
invertebrate in vitro, as a tissue-engineered microenvironment for the culture of parasites …

ABSTRACT A microfluidic system including a number of microfluidic devices having a first
perfusion path and a second separate perfusion path; the microfluidic devices each also
having a chamber containing a matrix, where the matrix surrounds at least one void whose
lumen is in fluidic connection exclu sively with the first perfusion path, where the at least one
void is populated with at least one cell type in such way that the cells are in direct contact
with the matrix; where the matrix is in fluidic connection exclusively with the second separate …