Biodiesel (methyl esters) production from vegetable oil has commonly used. This study has the objective to produce biodiesel from palm oil assisted microwaves system in reaction time and material feeding frequency variations. Heating using a microwave is a quick and simple because it just takes several minutes or seconds that the biodiesel process production takes place. In comparation with the conventional process that takes 75 minutes to get complete reaction, the microwave assisted process only takes 4 minutes. Irradiation time should be controlled to avoid overheating which could destroy some organic molecules and radiation power setting should not be too high because it can destroy organic molecules too. Microwaves used was 12.2 cm wave length with a frequency of 2.45 GHz. Transesterification performed using KOH catalyst as much as 1% of the raw material oil. Methanol and oil ratio is 6. Variations performed on the feeding frequency material (1.2, 4 and 8 times) and reaction time (2, 4, 6, 8 and 10 minutes). Microwave irradiation assisted for biodiesel production has more efficient in time and energy. It produces large enough yield that the best results feeding frequency of materials is 2 times in 10 minute that give 97.06% yield and 94,01% reaction conversion. Increasing reaction time will increase yield and conversion. Increasing feeding frequency of material will reduce the risk of overheating which can damage organic compounds but decrease yield and conversion.