This study was carried out to investigate the relationships milk somatic cell count (SCC) and pH in Holstein dairy cattle raised in a private dairy farm in Kirsehir province of Turkey. A total of 466 samples collected from 101 first lactation Holstein cows calved in 2015 year were used. Milk samples were collected monthly from individual cows from 30±15 to 150±15 days of lactation and three seasons (autumn, winter, spring and summer). The SCC data were classified into three classes corresponding to the content of SCC:< 100.000, 100.000-500.000 and> 500,000 cell/ml. The results showed that the effects of stage of lactation and sampling season on logSCC and pH were statistically important (P< 0.01). Log-SCC and pH values were increased with advancing stage of lactation. LogSCC was significantly higher during summer while pH was higher in spring. Cows with SCC greater than 500,000 cell/ml had higher pH values. To conclude, milk pH should be considered as a criterion for detection of SCC and mastitis.