Fidelity of DNA polymerases in DNA amplification.

P Keohavong, WG Thilly - Proceedings of the National …, 1989 - National Acad Sciences
… The conditions used for T4 (21) and for modified T7 (24) DNA polymerases were similar to
those described for Klenow fragment except that the concentrations of dNTP were increased

DNA polymerases for whole genome amplification: considerations and future directions

CD Ordóñez, M Redrejo-Rodríguez - International Journal of Molecular …, 2023 - mdpi.com
… have shown promise in modifying DNA polymerases to improve their performance in WGA
protocols and other applications. Overall, the role of DNA polymerases in WGA techniques is …

Evolution of thermophilic DNA polymerases for the recognition and amplification of C2-modified DNA

T Chen, N Hongdilokkul, Z Liu, R Adhikary, SS Tsuen… - Nature …, 2016 - nature.com
… We next explored the PCR amplification of C2ʹ-F-modified oligonucleotides. The initial
experiments suggested that PCR amplification was very efficient when any pair of the four …

Amplification efficiency of thermostable DNA polymerases

B Arezi, W Xing, JA Sorge, HH Hogrefe - Analytical biochemistry, 2003 - Elsevier
… the amplification efficiency of 10 different DNA polymerase … A number of modifications to the
basic PCR format including … in an attempt to increase amplification efficiency and specificity, …

Modified DNA polymerases for PCR troubleshooting

M Śpibida, B Krawczyk, M Olszewski, J Kur - Journal of applied genetics, 2017 - Springer
… for new DNA polymerases of natural origin or genetically modified ones to get better parameters
of the … Because there are many problems during amplification of different DNA samples, …

[PDF][PDF] Enhancement of PCR amplification yield and specificity using AmpliTaq gold [TM] DNA polymerase

T Moretti, B Koons, B Budowle - Biotechniques, 1998 - archive.gfjc.fiu.edu
… of AmpliTaq Gold™ DNA Polymerase to enhance the specificity and yield of amplification was
evaluated in a … A modified PCR protocol with a 67C annealing temperature (5) was used to …

Recent developments in the optimization of thermostable DNA polymerases for efficient applications

AR Pavlov, NV Pavlova, SA Kozyavkin… - Trends in …, 2004 - cell.com
… potential in genetically modified DNA polymerases, which … and accuracy of DNA amplification
applications essential to … of DNA polymerases is essential for their use in amplification

Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs

M de Vega, JM Lázaro, M Mencía… - Proceedings of the …, 2010 - National Acad Sciences
… of DNA synthesis by modifying the isothermal amplification enzyme … DNA polymerases
with those modified protocols will contribute to make φ29 DNA polymerase-based amplification

Alteration of enzymes and their application to nucleic acid amplification

K Yasukawa, I Yanagihara… - … Journal of Molecular …, 2020 - spandidos-publications.com
DNA topoisom-erase V to Taq polymerase increased the enzyme's stability and processivity
(13). The performances of DNA polymerasesModified family B DNA polymerases with a Pol

Structural insights into the processing of nucleobase-modified nucleotides by DNA polymerases

A Hottin, A Marx - Accounts of Chemical Research, 2016 - ACS Publications
… The DNA polymerase-catalyzed incorporation of modified nucleotides is employed in many
… by systematic enrichment of ligands by exponential amplification (SELEX). Recent studies …