Modulation of the interaction between human P450 3A4 and B. megaterium reductase via engineered loops
Chimerogenesis involving cytochromes P450 is a successful approach to generate
catalytically self-sufficient enzymes. However, the connection between the different
functional modules should allow a certain degree of flexibility in order to obtain functional
and catalytically efficient proteins. We previously applied the molecular Lego approach to
develop a chimeric P450 3A4 enzyme linked to the reductase domain of P450 BM3 (BMR).
Three constructs were designed with the connecting loop containing no glycine, 3 glycine or …
catalytically self-sufficient enzymes. However, the connection between the different
functional modules should allow a certain degree of flexibility in order to obtain functional
and catalytically efficient proteins. We previously applied the molecular Lego approach to
develop a chimeric P450 3A4 enzyme linked to the reductase domain of P450 BM3 (BMR).
Three constructs were designed with the connecting loop containing no glycine, 3 glycine or …
[引用][C] Modulation of the interaction between human P450 3A4 and B. Megaterium reductase via engineered loops
D Nardo, J Sheila - BBA-Proteins and Proteomics, 2017 - Elsevier BV
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