simplex sensu lato. This study was carried out to compare the amino acid sequences of
three major (Ani s 1, 2 and 12) and one minor (Ani s 9) Anisakis allergens between A.
simplex ss and Anisakis pegreffii. We found 2 (out of 163), 1 (out of 869), and 29 (out of 266)
amino acid variable sites for Ani s 1, 2, and 12, respectively. However, as both intra-and inter-
species variations were present at the same amino acid positions, no amino acid variations …