Molecular cloning and amino acid sequence of human 5-lipoxygenase.
5-Lipoxygenase (EC 1.13. 11.34), a Ca2+-and ATP-requiring enzyme, catalyzes the first two
steps in the biosynthesis of the peptidoleukotrienes and the chemotactic factor leukotriene
B4. A cDNA clone corresponding to 5-lipoxygenase was isolated from a human lung lambda
gt11 expression library by immunoscreening with a polyclonal antibody. Additional clones
from a human placenta lambda gt11 cDNA library were obtained by plaque hybridization
with the 32P-labeled lung cDNA clone. Sequence data obtained from several overlapping …
steps in the biosynthesis of the peptidoleukotrienes and the chemotactic factor leukotriene
B4. A cDNA clone corresponding to 5-lipoxygenase was isolated from a human lung lambda
gt11 expression library by immunoscreening with a polyclonal antibody. Additional clones
from a human placenta lambda gt11 cDNA library were obtained by plaque hybridization
with the 32P-labeled lung cDNA clone. Sequence data obtained from several overlapping …
[引用][C] Molecular cloning and amino acid sequence of human 5-lipoxygenase.
Molecular cloning and amino acid sequence of human 5-lipoxygenase. - Abstract - Europe
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