Molecular Detection of Histoplasma capsulatum var. capsulatum in Human Clinical Samples
A Bracca, ME Tosello, JE Girardini… - Journal of Clinical …, 2003 - Am Soc Microbiol
Journal of Clinical Microbiology, 2003•Am Soc Microbiol
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion
of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific,
being able to detect genomic material corresponding to less than 10 yeast cells without
cross-reaction against other bacterial or fungal pathogens.
of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific,
being able to detect genomic material corresponding to less than 10 yeast cells without
cross-reaction against other bacterial or fungal pathogens.
Abstract
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.
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