Materials and Methods: Fecal samples were obtained from 46 children with acute diarrhea
in a rural community during an episode of acute diarrhea, immediately after recovery from
diarrhea, and 3 months after recovery. DNA was extracted and quantitative polymerase
chain reaction using SYBR green and genus-and species-specific primers targeting 16S
rDNA were undertaken to quantitate the following groups of bacteria: Bifidobacterium spp.,
Bifidobacterium longum group, Bacteroides-Prevotella group, Bacteroides fragilis …

Abstract Background and Objective: The commensal bacterial flora of the colon may
undergo changes during diarrhea, owing to colonization of the intestine by pathogens and to
rapid intestinal transit. This study used molecular methods to determine changes in the
composition of selected commensal anaerobic bacteria during and after acute diarrhea in
children. Materials and Methods: Fecal samples were obtained from 46 children with acute
diarrhea in a rural community during an episode of acute diarrhea, immediately after …