Kinetic PCR analysis: real-time monitoring of DNA amplification reactions

R Higuchi, C Fockler, G Dollinger, R Watson - Bio/technology, 1993 - nature.com
… starting number of DNA targets. In this paper, we show that a kinetic analysis mode, in which
the level of amplified DNA is continuously monitored over the course of amplification, can …

Continuous fluorescence monitoring of rapid cycle DNA amplification

CT Wittwer, MG Herrmann, AA Moss… - Biotechniques, 2013 - Taylor & Francis
… with SYBR Green I show the temperature dependence of strand status during DNA
amplification (Figure 5). Early cycles appear identical, with a nonlinear increase in fluorescence at …

Simultaneous amplification and detection of specific DNA sequences

R Higuchi, G Dollinger, PS Walsh, R Griffith - Bio/technology, 1992 - nature.com
polymerase chain reaction (PCR) such that specific DNAamplification can be continuously
monitored in order to follow its progress. The ability to simultaneously amplify specific DNA

On-chip real-time monitoring of multiple displacement amplification of DNA

BB Bruijns, F Costantini, N Lovecchio… - Sensors and Actuators B …, 2019 - Elsevier
… -time monitoring of the multiple displacement amplification reaction (MDA) is presented. The
amplification and detection … The polymerase chain reaction (PCR) is widely used for DNA

Extreme PCR: efficient and specific DNA amplification in 15–60 seconds

JS Farrar, CT Wittwer - Clinical chemistry, 2015 - academic.oup.com
… by real-time optical monitoring. … PCR from human genomic DNA can be completed in
15–60 s. When paired with high-resolution melting analysis (26), DNA variants can be amplified

Detection and monitoring of virus infections by real-time PCR

F Watzinger, K Ebner, T Lion - Molecular aspects of medicine, 2006 - Elsevier
… to monitor the increasing amount of product at early time points during the PCR reaction. …
of PCR, when the amplification product first becomes detectable. Quantification by real-time …

PCR microfluidic devices for DNA amplification

C Zhang, J Xu, W Ma, W Zheng - Biotechnology advances, 2006 - Elsevier
DNA/RNA templates used in PCR microfluidics. It is noted, especially, that this review is to
help a novice in the field of on-chip PCR amplification to … related to on-chip PCR microfluidics. …

Electrical and electrochemical monitoring of nucleic acid amplification

T Goda, M Tabata, Y Miyahara - … in bioengineering and biotechnology, 2015 - frontiersin.org
PCR is a standard technique for DNA amplification. The … by a DNA polymerase with
exonuclease activity during PCR, … In DNA sequencing by double strand synthesis, Illmina ® DNA

Product differentiation by analysis of DNA melting curves during the polymerase chain reaction

KM Ririe, RP Rasmussen, CT Wittwer - Analytical biochemistry, 1997 - Elsevier
… fluoPolymerase chain reaction (PCR) amplification and rescence monitoring, combined with
melting curve PCR … - analysis, allows quantitative amplification and product tial procedures. …

Strand displacement amplification and the polymerase chain reaction for monitoring response to treatment in patients with pulmonary tuberculosis

TJ Hellyer, TW Fletcher, JH Bates… - Journal of Infectious …, 1996 - academic.oup.com
DNA amplification from use in therapeutic monitoring. For … been described for the
amplification of nucleic acid sequences … regarding its use to monitor the response of patients to …