In the Djerid palm groves, soils are often poor in mineral nutrients. Therefore, mycorrhizal symbiosis has a beneficial effect on date palm growth. But studies made on this symbiosis remains again very limited and even absent in Tunisia. In fact, 27date palms divided in to nine palm groves, have been studied for their rhizospheres and we can notice the presence in the roots of different date palm cultivars, four genera mycorrhizal fungi (Glomus, Acaulospora, Sclerocystis and Scutellospora). The predominant genus identified was Glomus. Eight species ofarbuscular mycorrhizal fungihave been recorded in all study sites: Glomus mosseae, Glomus constrictum, Glomu stortuosum, Glomus irregulare, Glomus sp., Acaulospora cavernata, Scutellospora calospora and Sclerocysti srubiformis. Their frequency and their rates vary depending on the sporulation sites and seasons. Seasonal variation of colonization by arbuscular mycorrhizal fungal (AMF) hyphae in all 8species has been characterized in vertical profiles and at different lateral distances from the date palm trunk. We can notice also that, the AMF colonization levels of field date palm roots were found to be according to the seasons. The peaks of colonization observed during autumn could be due to the sporadic rains that can activate fungus colonization and the tendency to associate depression with winter. The most important populations reached their maximum at the aplomb of the date palm crown and mainly 50 cm from the date palm trunk. The different AMF distributions were recorded at 20–40 cm soil depth; they refer to the most common roots of date palm.