Low stability of globular protein droplets in emulsion significantly limits their applications in
drug encapsulation, long-term storage, and controlled drug release. Here, a microfluidic flow-
focusing device was utilized to synthesize horseradish peroxidase (HRP)-loaded silk fibroin
microdroplets. The two immiscible streams of microfluidic flow-focusing were regenerated by
silk fibroin solution and a mixture of 95 wt.% sunflower oil and 5 wt.% span 80 as the
dispersed and continuous phases, respectively. In this study, the water-in-oil silk fibroin …