The reconstruction process of structured illumination microscopy (SIM) creates substantial
artefacts if the specimen has moved during the acquisition. This reduces the applicability of
SIM for live cell imaging, because these artefacts cannot always be recognized as such in
the final image. A movement is not necessarily visible in the raw data, due to the varying
excitation patterns and the photon noise. We present a method to detect motion by extracting
and comparing two independent 3D wide-field images out of the standard SIM raw data …

… Our algorithm locates motion and distinguishes artefacts from real high resolution cell
features. OCIS codes: (110.4280) Noise in … Structured illumination microscopy (SIM) is the
method of choice for high resolution live cell imaging because of its high acquisition speed
and comparably low phototoxicity. In addition, it is a well-established and commercially
available technique that can be used with any fluorophore. However, the necessary …
We believe that our algorithm has the potential to become a standard tool in SIM that …