Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly
enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this
review, we discuss multiplexed CRISPR technologies and describe methods for the
assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications
that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic …