Mycobacterial load assay

SH Gillespie, W Sabiiti, K Oravcova - Diagnostic bacteriology: methods and …, 2017 - Springer
… The mycobacterial load assay (MBLA) is a culture-free biomarker that aims at providing …
viable Mycobacterium tuberculosis in patient sputum. The assay monitors the molecular load of M…

Comparison of quantitative techniques including Xpert MTB/RIF to evaluate mycobacterial burden

RN van Zyl-Smit, A Binder, R Meldau, H Mishra… - PloS one, 2011 - journals.plos.org
… Three aliquots of each dilution were used in each of the 5-mycobacterial load determination
assays as described in the methods section. PCR-polymerase chain reaction, MGIT …

Molecular bacterial load assay, a culture-free biomarker for rapid and accurate quantification of sputum Mycobacterium tuberculosis bacillary load during treatment

I Honeyborne, TD McHugh, PPJ Phillips… - Journal of clinical …, 2011 - Am Soc Microbiol
… been proposed to describe the decline in bacterial load over the first 2 months of treatment,
… MBL assay-determined bacterial load in this study with the decline of bacterial load reported …

Molecular bacterial load assay concurs with culture on NaOH-induced loss of Mycobacterium tuberculosis viability

B Mtafya, W Sabiiti, I Sabi, J John… - Journal of clinical …, 2019 - Am Soc Microbiol
… Effective methods to detect viable Mycobacterium … We applied culture and the novel molecular
bacterial load assay (MBLA) … Compared to the bacterial loads of untreated controls, NALC-…

[HTML][HTML] Comparison of the Mycobacterium tuberculosis molecular bacterial load assay, microscopy and GeneXpert versus liquid culture for viable bacterial load …

HT Hai, DN Vinh, DDA Thu, NT Hanh, NH Phu… - Tuberculosis, 2019 - Elsevier
Molecular bacterial load assay (MBLA) rapidly quantifies viable Mycobacterium tuberculosis
(Mtb) and may be useful for monitoring treatment response and treatment efficacy. We …

Culture-Free Enumeration of Mycobacterium tuberculosis in Mouse Tissues Using the Molecular Bacterial Load Assay for Preclinical Drug Development

D Evangelopoulos, CM Shoen, I Honeyborne, S Clark… - Microorganisms, 2022 - mdpi.com
… Here we show that the MBL assay can be utilised in the enumeration of bacterial load from
infected as well as from drug-treated animal tissues. We have utilised lung tissues from three …

A tuberculosis molecular bacterial load assay (TB-MBLA)

W Sabiiti, BA Mtafya… - Journal of …, 2020 - research-repository.st-andrews.ac …
… Molecular bacterial load assay, a culture-free biomarker for rapid and accurate quantification
of sputum Mycobacterium tuberculosis bacillary load during treatment. Journal of Clinical …

Rapid Diagnosis of Mycobacterial Infections and Quantitation of Mycobacterium tuberculosis Load by Two Real-Time Calibrated PCR Assays

F Broccolo, P Scarpellini, G Locatelli… - Journal of clinical …, 2003 - Am Soc Microbiol
… Next, we matched the results of the AFB smear test and Mycobacterium culture with the
level of M. tuberculosis DNA load measured with the senX3-regX3 assay (either corrected or …

Molecular bacterial load assay versus culture for monitoring treatment response in adults with tuberculosis

B Said, L Charlie, E Getachew… - SAGE Open …, 2021 - journals.sagepub.com
load assay method, molecular expertise, and a well-equipped laboratory, tuberculosis
molecular bacterial load assay … , which usually involves culturing mycobacteria species on either …

Tuberculosis bacillary load, an early marker of disease severity: the utility of tuberculosis molecular bacterial load assay

W Sabiiti, K Azam, ECW Farmer, D Kuchaka, B Mtafya… - Thorax, 2020 - thorax.bmj.com
… and not all viable Mycobacterium tuberculosis (Mtb) bacilli are detected.5 6 Alternative more
rapid methods include mycobacterial DNA-detection assays but prolonged DNA survival in …