[HTML][HTML] Nano-rheology measurements reveal that the hydration layer of enzymes partially controls conformational dynamics
Z Alavi, A Ariyaratne, G Zocchi - Applied Physics Letters, 2015 - pubs.aip.org
For a typical (20 kD, 4 nm size) monomeric enzyme, more than 50% of the residues are at
the surface. The mechanics of these soft, heterogeneous nanoparticles was recently shown
to be viscoelastic. Here, we explore the contribution of the enzyme's surface to the
mechanics of the molecule. Nano-rheology provides sub-Å resolution measurements of the
reversible deformation of the enzyme subject to an oscillatory mechanical stress. We perturb
the surface of the enzyme by adding small amounts of DMSO (dimethyl sulfoxide), believed …
the surface. The mechanics of these soft, heterogeneous nanoparticles was recently shown
to be viscoelastic. Here, we explore the contribution of the enzyme's surface to the
mechanics of the molecule. Nano-rheology provides sub-Å resolution measurements of the
reversible deformation of the enzyme subject to an oscillatory mechanical stress. We perturb
the surface of the enzyme by adding small amounts of DMSO (dimethyl sulfoxide), believed …
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