We present nanostructured gold films ⎯ with complex plasmonic nanostructures present on the surface of the films ⎯ for enhanced SPR-based sensing and imaging of biomolecules attached to the surface of these films. We employed rigorous coupled wave analysis (RCWA) for simulating the nanostructured plasmonic gold films. In our simulations, surface plasmon polaritons were excited on the surface of the nanostructured gold films using the Kretschmann configuration. We observe that these nanostructured gold films show a significant enhancement in the sensitivity of SPR sensing and imaging of biomolecules as compared to planar gold films when optimal geometries and sizes of the plasmonic nanostructures (present on the surface of the gold film) are employed.