Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water
CR Arias, E Garay, R Aznar - Applied and Environmental …, 1995 - Am Soc Microbiol
CR Arias, E Garay, R Aznar
Applied and Environmental Microbiology, 1995•Am Soc MicrobiolA nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an
alternative to cultural methods by using universal primers flanking the V. vulnificus-specific
sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or
12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
alternative to cultural methods by using universal primers flanking the V. vulnificus-specific
sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or
12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
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