The article describes a new kind of textile transmission line which might be widely used, among others, in smart clothing. This line consists of a textile substrate in the form of fabric, sewn with paths made from electrically conductive fabric. The arrangement of electrically conductive paths in the line proposed makes it easy to obtain the possibility of impedance changes in a wide range of its values (potentially giving the possibility to obtain an impedance equal to 50 Ω), regardless of the type of fabric used as a substrate. The paper presents examples of measurement results of characteristic impedance of the line affecting its transmission properties. It also presents an impact study of the type of stitches fastening electrically conductive paths and the inaccurate creation of the line on the value of the characteristic impedance. The studies presented are based on real measurements and computer simulation using CST Studio software.