New dual monoclonal ELISA for measuring plasma osteopontin as a biomarker associated with survival in prostate cancer: clinical validation and comparison of …
Clinical chemistry, 2009•academic.oup.com
Background: A previously developed monoclonal/polyclonal ELISA (Mono/Poly) to detect
plasma concentrations of osteopontin (OPN) was shown to provide prognostic information in
breast, prostate, and other cancers. Here we describe the clinical validation of a new dual
monoclonal (Dual Mono) assay. We compared both assays with 4 assays that recognize
defined regions of OPN protein (dual polyclonal systems 5-1, 4-1, 4-3 and polyclonal-
monoclonal system 1-3). Methods: OPN sequences recognized by the monoclonal …
plasma concentrations of osteopontin (OPN) was shown to provide prognostic information in
breast, prostate, and other cancers. Here we describe the clinical validation of a new dual
monoclonal (Dual Mono) assay. We compared both assays with 4 assays that recognize
defined regions of OPN protein (dual polyclonal systems 5-1, 4-1, 4-3 and polyclonal-
monoclonal system 1-3). Methods: OPN sequences recognized by the monoclonal …
Abstract
Background: A previously developed monoclonal/polyclonal ELISA (Mono/Poly) to detect plasma concentrations of osteopontin (OPN) was shown to provide prognostic information in breast, prostate, and other cancers. Here we describe the clinical validation of a new dual monoclonal (Dual Mono) assay. We compared both assays with 4 assays that recognize defined regions of OPN protein (dual polyclonal systems 5-1, 4-1, 4-3 and polyclonal-monoclonal system 1-3).
Methods: OPN sequences recognized by the monoclonal antibodies that make up the Dual Mono ELISA were identified by Pepscan CLIPS™ analysis. Using the 6 ELISAs, we measured OPN in plasma from 66 patients with castration-resistant prostate cancer, and we assessed the ability of each assay to predict patient survival.
Results: The assays varied in measured plasma OPN concentrations, with median values ranging from 112 to 1740 μg/L, and ability to predict patient survival. By Cox univariable regression of survival by tertiles of OPN, the Mono/Poly and Dual Mono ELISAs had the highest log-rank χ2 values. After adjustment for risk factors independently associated with survival in our samples, OPN remained associated with survival only for the Mono/Poly and Dual Mono systems.
Conclusions: OPN plasma values varied significantly depending on the assay used. Only the Mono/Poly and Dual Mono systems were independently associated with survival in a population of men with castration-resistant prostate cancer. The availability of a clinically validated, dual monoclonal–based ELISA will provide consistent reagents for studies of OPN plasma concentrations in cancer and other pathologies.
Oxford University Press