The present work focuses on the analysis of cell cycle progression of Paracoccidioides
brasiliensis yeast cells under different environmental conditions. We optimized a flow
cytometric technique for cell cycle profile analysis based on high resolution measurements
of nuclear DNA. Exponentially growing cells in poor-defined or rich-complex nutritional
environments showed an increased percentage of daughter cells in accordance with the
fungus' multiple budding and high growth rate. During the stationary growth-phase cell cycle …

Introduction and Objectives: The control mechanisms that regulate cellular and nuclear
division during cell growth of the pathogenic fungus Paracoccidioides brasiliensis are still
poorly understood. Considering the multiple budding cellular division and multinucleate
nature of this fungus, one would expect a strict regulation of the cell cycle and the interaction
between DNA replication, nuclei segregation and budding. The main goal of this work was
to study nuclear andVor cellular division of P. brasiliensis yeast cells, focusing on the …