We propose a new affinity sorbent, matrix-linked histidine, for the large-scale purification of
proteins. A variety of proteins and certain peptides, each distinct from the other, were
purified. Immunoglobulin G from human placenta was chosen for a detailed study
concerning the effects of coupling, spacer-arm and other parameters. Multiple interactions
such as charge-transfer and other ionic reactions have been suggested to be responsible for
the interactions between proteins and ligand.