Non-thermal microbial inactivation by using supercritical carbon dioxide: Synergic effect of process parameters

EK Silva, VO Alvarenga, MA Bargas… - The Journal of …, 2018 - Elsevier
In this study, we evaluated the influence of CO 2 volume ratio (%) and its synergic effect with
the pressure (MPa), temperature (° C) and process time (min) on the inactivation of
Lactobacillus casei cells inoculated in apple juice (10 7 CFU/mL). The effects of process
variables on the logarithmic reduction of L. casei cell counts were estimated by a two-level
Plackett–Burman factorial design. A Box–Behnken design was subsequently employed to
evaluate the interaction between the different factors on the supercritical microbial …
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