Nuclear and cytoskeletal dynamics during oocyte maturation and development of somatic cell cloned pig embryos injected with membrane disintegrated donor cells
The objectives of this study were to characterize the nuclear and cytoskeletal changes of pig
oocytes during in vitro maturation (IVM) and the development of the reconstructed embryos
after injection with membrane intact or disintegrated donor cells. Cumulus-oocyte complexes
(COCs) were collected from abattoir ovaries by follicle (2–8mm) aspiration. In Experiment 1,
COCs were cultured in NCSU-23 medium for 0, 11, 22, 33, and 44h. Oocytes were fixed at
different time points for nuclear and cytoskeletal labeling. Forty-three percent and 75 …
oocytes during in vitro maturation (IVM) and the development of the reconstructed embryos
after injection with membrane intact or disintegrated donor cells. Cumulus-oocyte complexes
(COCs) were collected from abattoir ovaries by follicle (2–8mm) aspiration. In Experiment 1,
COCs were cultured in NCSU-23 medium for 0, 11, 22, 33, and 44h. Oocytes were fixed at
different time points for nuclear and cytoskeletal labeling. Forty-three percent and 75 …
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