Nucleic acid detection with CRISPR-Cas13a/C2c2

JS Gootenberg, OO Abudayyeh, JW Lee… - Science, 2017 - science.org
… Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen …
To simulate sample detection without nucleic acid purification, we measured detection of …

N 1-Methyladenosine detection with CRISPR-Cas13a/C2c2

Y Chen, S Yang, S Peng, W Li, F Wu, Q Yao… - Chemical …, 2019 - pubs.rsc.org
… In summary, the CRSIPR-Cas13a system exhibited limitless application in nucleic acid
detection. In this work, we first extended this system to the detection of epigenetic modification. In …

[PDF][PDF] Nucleic acid detection with CRISPR-Cas13a/C2c2

AJ Dy, J Joung, V Verdine, N Donghia, NM Daringer… - intbio.org
nucleic acid detection platform with attomolar sensitivity based on … To simulate sample
detection without nucleic acid purification, we measured detection of ssRNA 1 spiked into human …

CRISPR-Cas13a system: A novel tool for molecular diagnostics

L Zhao, M Qiu, X Li, J Yang, J Li - Frontiers in Microbiology, 2022 - frontiersin.org
… A novel CRISPR-based nucleic acid detection platform has been established, called the
SHERLOCK, which combines isothermal amplification that comprises recombinase polymerase …

Discovery, classification and application of the CPISPR-Cas13 system

X Nie, D Wang, Y Pan, P Lü… - Technology and Health …, 2024 - content.iospress.com
… developed 227 for mass detection of multiple viral variants by increasing the throughput of
the SHERLOCK method. Nano-droplets of nucleic acid detection reagent based on CRISPR …

CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges

J Zhou, Z Li, JS Olajide, G Wang - Heliyon, 2024 - cell.com
… Additionally, CRISPR/Cas-based nucleic acid detection … /Cas-based nucleic acid detection
methods and highlight some … the improvement of CRISPR/Cas-based nucleic acid testing. …

Development of CRISPR-mediated nucleic acid detection technologies and their applications in the livestock industry

X Zhang - Genes, 2022 - mdpi.com
… While CRISPR-RT [117] and CRISPR-DT [118] were developed to help scientists design
gRNAs for the CRISPR/Cas13a/C2c2 and CRISPR/Cas12a/Cpf1 systems, respectively. To …

[HTML][HTML] CRISPR Cas system: A strategic approach in detection of nucleic acids

FO Fapohunda, S Qiao, Y Pan, H Wang, Y Liu… - Microbiological …, 2022 - Elsevier
… role of CRISPR Cas system in detecting of nucleic acids, provided some examples where
CRISPR Cas proteins has been used in nucleic acids detection and stated the advantages of …

A chemical-enhanced system for CRISPR-Based nucleic acid detection

Z Li, W Zhao, S Ma, Z Li, Y Yao, T Fei - Biosensors and Bioelectronics, 2021 - Elsevier
… the detection power of CRISPR-based nucleic acid detection… /Cas13a-mediated nucleic acid
detection, here we examined … target amplification and CRISPR detection phases. We found …

[HTML][HTML] Design of crRNA to regulate MicroRNAs related to metastasis in colorectal cancer using CRISPR-C2c2 (Cas13a) technique

ST Houseini, F Nemati, A Sattari, M Azadeh… - Cell Journal …, 2023 - ncbi.nlm.nih.gov
Colorectal cancer (CRC) is the third most prevalent cancer with the second-highest mortality
rate worldwide. microRNAs (miRNAs) of cancer-derived exosomes have shown promising …