A new PCR primer set which enables one-step amplification of complete arthropod
mitochondrial genomes was designed from two conserved 16S rDNA regions for the long
PCR technique. For this purpose, partial 16S rDNAs amplified with universal primers 16SA
and 16SB were newly sequenced from six representative arthropods: Armadillidium vulgare
and Macrobrachium nipponense (Crustacea), Anopheles sinensis (Insecta), Lithobius
forficatus and Megaphyllum sp.(Myriapoda), and Limulus polyphemus (Chelicerata). The …

A primer set that can easily be used to amplify entire arthropod mitochondrial genomes with
one-step PCR was developed. Nucleotide sequences of 16SA-B regions from Armadillidium
vulgare (Crustacea), Macrobrachium nipponense (Crustacea), Lithobius forficatus
(Myriapoda), Megaphylum sp.(Myriapoda) and Limulus polyphemus (Chelicerata) were
newly determined prior to the designing of the long PCR primers. Four primers were
designed from the most conserved regions in the sequence alignment of 16SA-B regions …