Osteoclasts are multinucleated giant cells that differentiate from precursors of the monocyte-
macrophage lineage. We used receptor activator of NF-kappa B ligand (RANKL) and
macrophage colony stimulating factor (M-CSF) to differentiate authentic human osteoclasts
from peripheral blood mononuclear cells (PBMCs). We previously described a series of
genes that are strongly regulated by RANKL. Here, we provide a set of reliable quantitative
real-time PCR based assays of RANKL regulated genes as reference genes that may prove …