Sampling procedures and diagnostic protocols were optimized for accurate diagnosis of
Cassava brown streak virus (CBSV)(genus Ipomovirus, family Potyviridae). A cetyl trimethyl
ammonium bromide (CTAB) method was optimized for sample preparation from infected
cassava plants and compared with the RNeasy plant mini kit (Qiagen) for sensitivity,
reproducibility and costs. CBSV was detectable readily in total RNAs extracted using either
method. The major difference between the two methods was in the cost of consumables …