fermentation (SSF) using Bacillus subtilis natto (B. s. natto) to overcome the limitations of
SBM usage in aquafeed. The response surface methodology (RSM) was employed to
explore the relationships of fermentation conditions, such as temperature, time, water-
substrate ratio, and layer thickness, on the degree of protein hydrolysis (DH) and the crude
protein (CP) content. The optimum conditions for achieving the higher DH (15.96%) and CP …