Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development

PI Gangashetty, SH Belliappa, N Bomma… - Plant Methods, 2024 - Springer
PI Gangashetty, SH Belliappa, N Bomma, V Kanuganahalli, SB Sajja, S Choudhary
Plant Methods, 2024Springer
Background The challenge of pigeonpea breeding lies in its photosensitivity and seasonal
specificity. This poses a problem to the breeder, as it restricts to single generation
advancement in a year. Currently, the cross to cultivar gap is twelve to thirteen years
resulting in a limited number of varietal releases over the past six decades. Shortening the
breeding cycle was need of the hour, unlikely achieved by conventional breeding. To
overcome these hindrances speed breeding was a necessary leap. An experiment was …
Background
The challenge of pigeonpea breeding lies in its photosensitivity and seasonal specificity. This poses a problem to the breeder, as it restricts to single generation advancement in a year. Currently, the cross to cultivar gap is twelve to thirteen years resulting in a limited number of varietal releases over the past six decades. Shortening the breeding cycle was need of the hour, unlikely achieved by conventional breeding. To overcome these hindrances speed breeding was a necessary leap. An experiment was planned to optimize the speed breeding coupled with single seed descent and seed or pod chip-based genotyping to shorten the breeding cycle in pigeonpea at ICRISAT, Hyderabad. Monitored photoperiod, light wavelength, temperature and crop management regime were the indicators attributing to the success of speed breeding.
Result
A photoperiod of 13 h: 8 h: 13 h at vegetative: flowering and pod filling stages is ideal for shortening the breeding cycle. Broad spectrum light (5700 K LED) hastened early vegetative growth and pod formation. Whereas far-red (735 nm) light favoured early flowering. A significant difference between the photoperiods, genotypes as well as photoperiod x genotype interaction for both days to flowering and plant height was noted.
Conclusion
The optimized protocol serves as a road map for rapid generation advancement in pigeonpea. Deploying this protocol, it is possible to advance 2–4 generations per year. The breeding cycle can be reduced to 2–4 years which otherwise takes 7 years under conventional breeding. Single Seed Descent and seed or pod chip-based genotyping for early generation marker assisted selection, strengthened the precision of this technique aiding in high throughput line development.
Springer
以上显示的是最相近的搜索结果。 查看全部搜索结果