The 1, 3-dinitroglycerin is an important compound to synthesize glycidyl nitrate, monomer of polyglicidyl nitrate (PGN). PGN is the most energetic polymer which can be used as binder of propellant. This compound was produced from the nitration of glycerol with nitric acid. This paper aims to obtain optimum conditions nitration of glycerol with nitric acid. The reaction was run in a flask equipped with a nitrogen purge for stirring. Nitration of glycerol to 1, 3-dinitroglycerin was studied in the temperature range of 10-30 0C, the molar ratio of nitric acid to glycerol 1/1 to 7/1 and nitric acid concentration of 69%.%. From experiments it is known that the optimum conditions of nitration of glycerol with nitric acid is a reaction temperature of 20 0C, the mole ratio of nitric acid/glycerol 5/1 and the concentration of nitric acid 69%.