[PDF][PDF] PCR-Based Detection of a Hyperparasitoid in Cotton Mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

M Ashfaq, S Akhtar, S Akhtar, M Masood - Pakistan J. Zool, 2017 - academia.edu
M Ashfaq, S Akhtar, S Akhtar, M Masood
Pakistan J. Zool, 2017academia.edu
Materials and methods Aenasius bambawaleiis being multiplied on mealybug host for field
releases in the mass-rearing laboratory of the Directorate of Entomology, AARI, Faisalabad,
at controlled temperature (28±2 C) and humidity (70±5% RH)(Hameed et al., 2012).
Presence of the hyperparasitoid was noticed in early summer of 2010 in the rearing cages
when instead of A. bambawalei another wasp was found emerging from the mealybug
mummies. The emerging wasps were collected and identified using taxonomic literature and …
Materials and methods
Aenasius bambawaleiis being multiplied on mealybug host for field releases in the mass-rearing laboratory of the Directorate of Entomology, AARI, Faisalabad, at controlled temperature (28±2 C) and humidity (70±5% RH)(Hameed et al., 2012). Presence of the hyperparasitoid was noticed in early summer of 2010 in the rearing cages when instead of A. bambawalei another wasp was found emerging from the mealybug mummies. The emerging wasps were collected and identified using taxonomic literature and keys developed by Hayat and Verma (1980) and Hayat (1998). Based on the key characters including number of antennal segments, head structure and setae, presence of white band across T1 of gaster, wing venations, and tibial segments, the wasp was identified to the species,
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