[HTML][HTML] PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent

M Tabatabaei, MR Zakaria, RA Rahim… - Electronic Journal of …, 2009 - SciELO Chile
16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with
polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning
electron microscopy and denatured gradient gel electrophoresis were used to reveal the
distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill
effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted
followed by restriction fragment length polymorphism (RFLP) for presumptive identification of …

PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent.

MT Meisam Tabatabaei, MRZ Mohd Rafein Zakaria… - 2009 - cabidigitallibrary.org
Abstract 16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with
polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning
electron microscopy and denatured gradient gel electrophoresis were used to reveal the
distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill
effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted
followed by restriction fragment length polymorphism (RFLP) for presumptive identification of …
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