polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning
electron microscopy and denatured gradient gel electrophoresis were used to reveal the
distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill
effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted
followed by restriction fragment length polymorphism (RFLP) for presumptive identification of …