Efforts have been concentrated on developing alternative methods of enzyme purification
that are less costly and highly efficient. In this work, we evaluated three different methods for
lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate,
ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethylene glycol
(PEG) and potassium phosphate; and direct immobilization. It was impossible to obtain
stable precipitates of the enzyme due to the low concentration of total protein and the …