The high intensity of yellowfin tuna (Thunuus albacares) juvenils in the Tomini bay has occurred after purse seine operated in this area. The aim of the research on “Exploitation of juvenile yellowfin tuna in Tomini Bay” was to give a clear feature on yellowfin tuna juvenile exploitation by hand line and purse seine in the Tomini Bay. The research was conducted in 2007 and 2010. All samples of yellow fin tuna were caught by hand line and purse seine which were operated in the Tomini Bay. The result showed that juvenile yellowfin tuna were caught by hand line was about 88.11% from the total catch of hand line, while the yellowfin tuna juvenils caught by purse seine was 100%. Length at first capture of yellowfin tuna by handline and purse seine were 31.6 cm FL and 30 cm. Fishing juvenile yellowfin tuna occurs every month. Moreover, that condition was showing and giving the indication that the exploitation of juvenile yellowfin tuna was very high that may endanger their sustainability.