The infiltration of macrophage cell on wound healing process has important role to release a number of cytokines and synthesize extracellular matrix. The aim of this study was to account the the expression of macrophage cell on wound healing process of dental extraction in Rattus norvegicus for 3 and 4 days using chitosan gel with different molecular weight. Rattus nornegicus strain wistar male, aged 8-16 weeks, divided into 3 groups, namely group 1 which given chitosan gel 1% with high molecular weigh and high viscosity: group II which given chitosan gel 1% with low molecular weight and low viscosity: group III as control which were not given chitosan gel. Chitosan gel 1% were applied into the socket of dental extraction. Rat was decaputated 3 and 4 days after chitosan gel application and the jaw in the treated regions and control group were cut for immunohistochemical examination using macrophage cell monoclonal antibody to observethe expression of macrophage cell. Data were analyzed using ANOVA test. The expression of macrophage cells were found higher in the group which given chitosan gel 1% with high molecular weight. The result showed significant differences in expression of macrophage cell for 3 and 4 days observation compared to control group (p< 0, 05). The application chitosan gel 1% with high molecular weight and high viscosity might stimulates macrophages cells on wound healing process of dental extraction.