The biodiesel obtained from the seeds of Callophyllum innophyllum has been analyzed for its physico chemical properties. The oil yield potential of Callophyllum seed and the biodiesel properties of the oil were studied in order to know the feasibility of the oil as a biofuel. The physico-chemical properties of the biodiesel produced by blending with the conventional diesel were evaluated and compared with that of diesel. The physico-chemical properties assessed includes, specific gravity, density, viscosity, flash point, fire point, cloud point, pour point, smoke point, pH, carbon residue, ultrasonic parameters, average molecular weight, saponification value, acid value, and iodine value. The results revealed that there is a significant difference between the physico-chemical properties of biodiesel and petro diesel.