The objective of this study was to determine the antimicrobial activity of methanol, chloroform and aqueous extracts of Microcos Paniculata leaves against some gram positive and gram negative bacteria such as B. subtilis, B. cereus, S. typhi, V. cholerae, P. mirabilis, E. coli, S. aureus, Serratia spp., Erwinia spp., Pseudomonas spp., Salmonella spp., Shigella boydii, B. megaterium. The in vitro antimicrobial activity was performed by agar disc diffusion method. However, maximum antimicrobial activity of the plant extracts was found against gram negative bacteria. Among the plant extracts, methanolic extract of Microcos Paniculata leaves showed highest zone of inhibition (27mm) against Salmonella spp. So, the Microcos Paniculata leaves may be a source of antimicrobial agent.