real-time RT-PCR participated in this evaluation. At each laboratory, 100 nasopharyngeal
swab samples including 10 positive samples were used to create 5-and 10-sample pools
with one positive sample in each pool. RNA extraction and real-time RT-PCR for SARS-CoV-
2-specific E gene target were performed for individual positive samples as well as pooled
samples. Concordance between individual sample testing and testing in the 5-or 10-sample …