Pooled testing for COVID-19 diagnosis by real-time RT-PCR: A multi-site comparative evaluation of 5-& 10-sample pooling
I Praharaj, A Jain, M Singh… - Indian Journal of …, 2020 - journals.lww.com
Methods: Ten virus research and diagnostic laboratories (VRDLs) testing for COVID-19 by
real-time RT-PCR participated in this evaluation. At each laboratory, 100 nasopharyngeal
swab samples including 10 positive samples were used to create 5-and 10-sample pools
with one positive sample in each pool. RNA extraction and real-time RT-PCR for SARS-CoV-
2-specific E gene target were performed for individual positive samples as well as pooled
samples. Concordance between individual sample testing and testing in the 5-or 10-sample …
real-time RT-PCR participated in this evaluation. At each laboratory, 100 nasopharyngeal
swab samples including 10 positive samples were used to create 5-and 10-sample pools
with one positive sample in each pool. RNA extraction and real-time RT-PCR for SARS-CoV-
2-specific E gene target were performed for individual positive samples as well as pooled
samples. Concordance between individual sample testing and testing in the 5-or 10-sample …
以上显示的是最相近的搜索结果。 查看全部搜索结果